Exhaled isopropanol: new potential biomarker in diabetic breathomics and its metabolic correlations with acetone
نویسندگان
چکیده
Concomitant findings of acetone (ACE) and isopropanol (IPA) in blood and other biological samples have been reported in diabetic decedents and clinic cases, but exhaled IPA has rarely been studied in breath research. This study aimed to investigate expression of exhaled IPA in diabetes and further explore the correlations between exhaled IPA and ACE, and to evaluate diabetes diagnostic applicability of exhaled IPA in combination with ACE. Exhaled breath samples at one time point from 85 type 2 diabetic patients and 56 healthy controls, and from four healthy individuals after ketogenic diet experiments were analyzed by gas chromatography mass spectrometry coupled with solid phase micro-extraction technique. Concentrations of exhaled IPA in the diabetic group (mean 85.44 ppbv) were significantly higher than those in the healthy group (mean 17.99 ppbv, p < 0.001). Ketogenic diet experiments showed that both IPA and ACE levels were elevated after keto-meals when under fat-consuming metabolic states. And they shared a similar changing pace, even though there was no linear relationship between IPA and ACE in terms of concentrations. The Spearman Correlation Coefficient between exhaled IPA and ACE in diabetes was 0.66, which indicated that IPA and ACE were metabolically correlated. ROC curve analysis showed that IPA possessed promising discriminatory ability (AUC 0.86) with a sensitivity of 75.3% and a specificity of 85.7% for diabetes diagnosis. This research indicates that endogenously produced IPA is a valuable biomarker for noninvasive diabetes diagnosis in breathomics analysis. This work also addressed the speculation that IPA can be metabolized from ACE via the reversible action of alcohol dehydrogenase.
منابع مشابه
Breathomics for Gastric Cancer Classification Using Back-propagation Neural Network
Breathomics is the metabolomics study of exhaled air. It is a powerful emerging metabolomics research field that mainly focuses on health-related volatile organic compounds (VOCs). Since the quantity of these compounds varies with health status, breathomics assures to deliver noninvasive diagnostic tools. Thus, the main aim of breathomics is to discover patterns of VOCs related to abnormal meta...
متن کاملMonitoring of oxidative and metabolic stress during cardiac surgery by means of breath biomarkers: an observational study
BACKGROUND Volatile breath biomarkers provide a non-invasive window to observe physiological and pathological processes in the body. This study was intended to assess the impact of heart surgery with extracorporeal circulation (ECC) onto breath biomarker profiles. Special attention was attributed to oxidative or metabolic stress during surgery and extracorporeal circulation, which can cause org...
متن کاملElevated exhaled acetone concentration in stage C heart failure patients with diabetes mellitus
BACKGROUND Breath acetone is a noninvasive biomarker of heart failure; however, its significance in heart failure patients with diabetes mellitus has yet to be clarified. The objective of this study is to investigate whether exhaled acetone concentration is a noninvasive biomarker in heart failure patients with diabetes mellitus. METHODS This study prospectively included 35 diabetic patients ...
متن کاملMicropreconcentrator in LTCC Technology with Mass Spectrometry for the Detection of Acetone in Healthy and Type-1 Diabetes Mellitus Patient Breath
Breath analysis has long been recognized as a potentially attractive method for the diagnosis of several diseases. The main advantage over other diagnostic methods such as blood or urine analysis is that breath analysis is fully non-invasive, comfortable for patients and breath samples can be easily obtained. One possible future application of breath analysis may be the diagnosing and monitorin...
متن کاملChange of Exhaled Acetone Concentration in a Diabetic Patient with Acute Decompensated Heart Failure.
In heart failure patients, exhaled acetone concentration, a noninvasive biomarker, is increased according to heart failure severity. Moreover, exhaled acetone concentration is also known to be affected by diabetes mellitus. However, there have been no reports on exhaled acetone concentration in heart failure patients with diabetes mellitus. A 77-year old man was admitted to our hospital with ac...
متن کامل